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A cDNA encoding the heavy-chain of horse serum transferrin has been obtained. The sequenced segment codes for a polypeptide of 871 amino acids (aa), with a calculated M(r) of 98,645. The following aa sequence has been determined: 66, 71, 75, 80, 86, 95, 100, 103, 107, 108, 112, 114, 117, 118, 123, 130, 134, 138, 141, 143, 144, 145, 147, 150, 151, 159, 161, 165, 167, 168, 172, 174, 175, 178, 186, 190, 192, 193, 194, 205, 207, 208, 212, 223, 230, 231, 234, 237, 238, 241, 249, 250, 251, 258, 259, 272, 281, 290, 298, 302, 306, 307, 315, 320, 333, 335, 344, 350, and 354. Some of these aa are highly homologous to human and sheep serum transferrin sequences. About 10 out of the 50 most N-terminally-sequenced aa are identical with those of the human transferrin sequence. The amino acid sequence is about 95% homologous with that of human serum transferrin. This is the first molecular cloning of a horse transferrin cDNA, to our knowledge. As a step towards the design of a horse transferrin gene library, the sequence obtained has been used to determine the position of overlapping start and stop codons, and the 5′ and 3′ non-coding regions.Product Information
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City Hall, originally the Center for Progress, is a skyscraper located in downtown Los Angeles, California, at the corner of East 7th Street and Los Angeles Street (formerly Figueroa Street). It is the current seat of the city government of Los Angeles. The building is located within the federal building and post office modernization project (FPB). Construction began in 2010 and was completed in 2013.
The original structure at this location was a pair of low-rise government buildings built in 1919 and 1921. The 1922 pair was demolished for the current building. The large sign located at one corner is the only relic of the old City Hall. Plans were first drafted in 1921. The Los Angeles City Council authorized the construction of a new city hall to its center at the corner of Figueroa Street and 7th Street in April 1923. A decision to choose plans by Rowland Lee Todd, a prominent Los Angeles architect, was announced in October 1923. The new building’s cost was estimated at $8.4 million, $1.1 million more than the original estimate.
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